Doktorsavhandlingar / Doctoral Theses - C2 (MBB): Recent submissions
Now showing items 161-180 of 308
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Medium chain dehydrogenases/reductases : alcohol dehydrogenases of novel types
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2003-10-03)The medium chain dehydrogenase/reductase (MDR) superfamily is separated into at least eight families, of which the dimeric alcohol dehydrogenase (ADH) is one. Including species variants, close to 1000 MDR forms have been ... -
Survival and differentiation of central noradrenergic neurons
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2002-08-09)About half of all central noradrenergic (NA) neurons reside in the locus coeruleus (LC). Projections from the LC reach target regions throughout the entire brain and regulate a variety of functions, such as mood, vigilance, ... -
Cloning and characterization of human glutaredoxins
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2002-05-24)Reactive oxygen species, ROS, are generated in cells during aerobic metabolism. High and sustained levels of ROS pose a serious threat to all major cellular components, DNA, proteins mid lipids whereas controlled and ... -
Organohalogen contaminants in humans with emphasis on polybrominated diphenyl ethers
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2002-02-08)The occurrence and distribution of organohalogen compounds, including polybrominated diphenyl ethers (PBDEs), in humans was investigated. For this purpose, the previously used method for analysis of organochlorine compounds ... -
Human truncated thioredoxin (Trx80) as a novel mitogenic cytokine for white blood cells
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-11-27)Thioredoxin (Trx) is a 12 kDa protein present in all species with a well-conserved active site sequence comprising -Cys-Gly-Pro-Cys-, which catalyzes oxido-reductase reactions. Trx regulates the activity of transcription ... -
Crystallographic studies of gluconate kinase
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-11-23)The first steps of gluconate metabolism in Escherichia coli are the transport of gluconate into the cell by a gluconate transport system, followed by phosphorylation to gluconate-6- phosphate by gluconate kinase. There ... -
Evolution and structure of leukotriene A4 hydrolase
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-10-12)Leukotriene (LT) B4 is a potent leukocyte chemotactic and aggregating agent involved in a variety of inflammatory and allergic disorders. The enzyme leukotriene A4 hydrolase catalyzes the hydrolysis of LTA4 into this ... -
The transaldolase family : structure, function and evolution
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-11-02)The transaldolase gene family includes representatives from all kingdoms of life and the encoded enzymes are important for carbohydrate metabolism. The family can be divided into two subfamilies; Classical transaldolases ... -
GDNF family ligands and neural stem cells in Parkinson's disease
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-10-04)Parkinson's disease (PD) is a very common neurodegenerative disorder, characterized by a progressive degeneration of mainly nigrostriatal and mesolimbic dopaminergic neurons leading to tremor, rigidity and hypokinesia, the ... -
Expression and function of GDNF family ligands and receptors in the nervous system
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-08-24)The search for novel neurotrophic factors promoting dopamine uptake and the survival of central dopaminergic neurons led to the discovery of a novel neurotrophic factor termed glial cell-line derived neurotrophic factor ... -
Genetic basis of congenital nephrotic syndrome and generation of an animal model
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-05-11)Congenital nephrotic syndrome, NPHS1, is a disease highly enriched in Finland with incidence of 1:8200 births. The disease is characterized by massive proteinuria, go % of which is albumin, starting in utero, large placenta, ... -
Mass spectrometry in protein structure analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-04-20)Mass spectrometry is an important analytical tool in biological and biochemical research. The speed, accuracy and sensitivity is unmatched by conventional analytical techniques. Identification of proteins and characterisation ... -
Protein and lipid interactions of mammalian antibacterial peptides
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-03-09)Gene-encoded antibacterial peptides are multifunctional effector molecules and play an important role in host innate immunity. Upon stimulation, the mature active peptides are released from inactive precursors. Cathelicidins ... -
Neurotrophic factors and neuronal plasticity
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2001-01-19)The neurotrophic factors were originally discovered because of their ability to rescue neurons during a period of naturally occurring programmed cell death. This gave rise to the neurotrophic factor hypothesis which states ... -
Pulmonary surfactant proteins B and C : molecular organisation and involvement in respiratory disease
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-11-23)Lung surfactant is a complex mixture of phospholipids and proteins with the main function to reduce the surface tension at the alveolar air/liquid interface. Surfactant protein B (SP-B) and C (SP-C) are hydrophobic but ... -
Chemical and mass spectrometrical methods in protein analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-11-24)Sensitive analytical methods are essential in protein studies because of frequently encountered limited sample availability. There is a constant need for optimization of sample preparation and analytical procedures for ... -
Detecting the dynamics of single biomolecules
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-10-13)This thesis is concerned with the detection of single biomolecules. Through detection of individual molecules it can be clarified whether a behavior which has been observed in a population is representative for all molecules. ... -
Structure and function of MMP-2 and its inhibitor TIMP-2
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-10-06)Animal cells secrete various enzymes capable of degrading the extracellular matrix. One of the most studied groups of these are the matrix metalloproteinases (MMPs). This group of zinc containing endopeptidases consists ... -
Palmitoylation and amyloid fibril formation of lung surfactant protein C
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-09-22)Lung surfactant is a mixture of lipids and a few proteins, of which surfactant proteins (SP)-B and SP-C are lipophilic. Surfactant is essential for the reduction of surface tension at the alveolar air/liquid interface. The ... -
Development of new NMR techniques and structural characterization of complexes between the N-terminal domain of the E. coli arginine repressor and operator DNA
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-09-15)This thesis includes the development of new solution NMR methods for the study of biomolecules and the characterization of complexes between the N-terminal domain of the E. coli arginine repressor (ArgR-N) and operator ...