Doktorsavhandlingar / Doctoral Theses - C2 (MBB): Recent submissions
Now showing items 141-160 of 308
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Development of new NMR techniques and the structure of the N-terminal domain of Escherichia coli DnaB helicas
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-02-26)New techniques applicable in biomolecular nuclear magnetic resonance (NMR) spectroscopy were developed. The three-dimensional structure of the N-terminal domain of Escherichia coli DnaB helicase was determined at atomic ... -
Mass spectrometry in peptide and tumor marker analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-03-05)Integration of preparative protein and peptide separations with analytical techniques, in particular mass spectrometry (MS), is important. This thesis describes preparation via capillary electrophoresis and two-dimensional ... -
Glutaredoxin 3 from Escherichia coli : NMR structure analysis and structural aspects of the enzymatic mechanism
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1998-10-30)The structure of Escherichia coli glutaredoxin 3 (Grx3) was investigated using NMR spectroscopy in combination with isotope labeling. Nearly complete sequence specific assignment were obtained for the reduced Grx3 using a ... -
Studies on the structure, function and expression of phospholipase A2
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1998-10-29)Phospholipase A2 (PLA2) enzymes are widely distributed and the enzymes possess different functions such as a role in digestion and inflammation. PLA2 catalyzes the hydrolysis of the sn-2 acylester bond of phospholipids, ... -
Molecular aspects of proinsulin C-peptide interactions
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2006-05-10)Insulin biosynthesis in the beta-cells of the pancreas begins with the generation of preproinsulin, which is converted to proinsulin. Proinsulin is cleaved into equimolar amounts of insulin and connecting peptide (C-peptide), ... -
Wnt signalling in the development of ventral midbrain dopaminergic neurons
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-12-24)Dopaminergic (DA) neurons in the ventral midbrain (VM) are one of the major cell types lost in Parkinson's disease (PD). Proof-of-principle exists for cell replacement therapies for PD, but wider application is halted by ... -
Native protein mass spectrometry of nuclear receptor-ligand and enzyme-substrate complexes
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-11-05)Nuclear receptors (NRs) are transcription factors that are activated by the binding of low molecular weight ligands. The NR superfamily includes receptors for steroid hormones, anti-inflammatory and lipid-lowering drugs ... -
Leukotriene A4 hydrolase : studies of structure-function relationships by site-directed mutagenesis and X-ray crystallography
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-10-15)Inflammation is the pathophysiological mechanism by which an organism clears and rebuilds a site of infection or injury. It is also the characteristic feature of a multitude of severe diseases, with the potential of ... -
Molecular mechanisms in amyloid fibril formation
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-05-21)This thesis concerns investigations on molecular processes and interactions that lead to amyloid fibril formation. Pulmonary surfactant protein C (SP-C), and a synthetic analogue thereof, SP-C(Leu), have similar properties ... -
Metalloproteinases in development and disease
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-08-06)Metalloproteinases are Zn-dependent proteases composed of five superfamilies. One important subfamily is the matrix metalloproteinases (MMPs) that process various components of the extracellular matrix (ECM) and non-ECM ... -
Development of a novel methodology for the synthesis of oligonucleo-peptide conjugates
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-05-20)Peptide-oligonucleotide conjugates constitute a new class of antisense molecules that exhibit improved biological activity over standard oligonucleotide analogues. Currently, these oligomeric bioconjugates are prepared by ... -
Microfluidic electrocapture technology in protein and peptide analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-05-19)After sequencing the genomes of several organisms, science in the postgenomic era now aims at a thorough study of the proteins present in a given tissue or organism. Since this task requires an enormous analytical effort, ... -
Sample preparation and mass spectrometry in proteome studies
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-05-07)Proteome studies aim to link gene to function. Instead of screening for function in a specific protein as in classical protein chemistry, proteins are now screened against a specific phenotype, disease or metabolic state. ... -
Membrane protein topology : prediction, experimental mapping and genome-wide analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-05-12)Membrane proteins constitute a large and important class of proteins. They are responsible for many crucial processes in living cells, and about 25% of all proteins encoded by a typical genome are predicted to be membrane ... -
Purification, structure and function of bioactive peptides
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-04-02)Peptides are vitally important molecules and many evoke cellular responses. The completion of several genome sequencing projects has revealed a number of new genes. However, as functional peptides often contain posttranslational ... -
Functional genomics at the interface of protein expression and biophysical analysis
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-02-18)Protein characterization is central to any functional genomics effort, hence protein expression and purification play fundamental roles in these initiatives. In this study strategies for protein expression in E. coli were ... -
Structural insights into glycoprotein transport and viral escape
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-02-16)The correct trafficking of proteins through the secretory pathway is essential for the maintenance of cellular functions. Increasing evidence suggests that at least for some glycoproteins, export from the endoplasmic ... -
Specificity of neurotrophins in the nervous system : a genetic approach to determine receptor engagement by neurotrophins
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2004-01-02)The classical neurotrophic factor hypothesis describes the neurotrophins as retrograde signalling factors supporting the survival of postmitotic neurons during the development. Knock-out mice for the neurotrophins and/or ... -
Expression of thioredoxin reductase 1 in mammalian cells with regulation by the core promoter and use of alternative splice variants
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2003-11-24)Reactive oxygen species are generated in cells as by-products of aerobic metabolism. To protect from damaging effects the cells possess several antioxidant systems and one of the most important is the thioredoxin system. ... -
Characterization of NPY receptors Y1, Y2 and Y5 expression and function in vivo
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2003-11-25)The general aim of these studies was to further elucidate the role of NPY receptors in the regulation of the food intake and energy homeostasis, nociception, and inflammation. We have utilized both normal and genetically ...