Doktorsavhandlingar / Doctoral Theses - C2 (MBB): Recent submissions
Now showing items 121-140 of 308
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Proteomics of neurodegenerative diseases using novel online isoelectric point separation
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-11-16)The work presented in this thesis describes an instrument, developed for separation of proteins and peptides based on corresponding isoelectric point (pI) values, to empower mass spectrometry-based proteomics analysis. The ... -
Study of the mechanisms behind the additive effect of neoadjuvant castration on radiotherapy for prostate cancer
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-10-23)Castration improves responses to radiotherapy (RT) in prostate cancer with unknown mechanism. An understanding of what happens at the cellular and molecular level in prostate cancer cells, while reducing their access to ... -
Elucidation of thioredoxin reductase 1 as an anticancer drug target
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-08-20)Cancer constantly finds ways to survive, so we must find new ways to stop it. A major attribute of cancer cells is increased oxidative stress, occurring in the form of reactive oxygen species (ROS). Basal ROS generation ... -
Mammalian thioredoxin reductase 1 in antioxidant defense, regulation of adipocyte differentiation and as an anticancer drug target
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-08-03)Reactive oxygen species (ROS) are oxygen containing reactive molecules generated as by-products of cellular metabolism. At physiological concentrations, ROS acts as secondary messengers in cellular signaling transduction, ... -
Deciphering ret signalling in cell biology and development
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-05-27)The rearranged during transformation (RET) tyrosine kinase regulates a plethora of biological processes such as cell survival, proliferation and migration and is essential for the normal development of several organs ... -
Structural and functional studies of nutrient and drug uptake systems
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-05-25)Orally ingested nutrients and drugs are selectively absorbed from our small intestines into the bloodstream through various membrane-integrated transporters. The present study focuses mainly on a specific absorption ... -
Ion homeostasis in control of stem cell proliferation
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-01-23)The ion homeostasis is critically maintained in all cells by a combined effort from various ion channels, transporters, pumps, and gap junctions. Ion fluxes are essential in excitable cells as they regulate the excitability ... -
Induction of epithelial-mesenchymal transition (EMT) in women's cancer : protective role of differentiation factors
(Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2015-01-07)Metastatic spread of cancer cells to vital organs is the predominant cause of death among women suffering from breast and ovarian cancer, and invasive cancer cells are in many cases resilient to standard drugs used in the ... -
Selenium in mammalian thioredoxin reductase
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-05-30)Thioredoxin reductase (TrxR) is a major enzyme in regulating intracellular redox status. The enzyme from several mammalian tissues has been purified, showing higher molecular weight and a much broader substrate specificity ... -
Isolation and characterization of lipid-associated and neurosecretory polypeptides
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-05-19)Lipid-interacting proteins play important roles in all living organisms. This thesis focuses on isolation and characterization of an enzyme in the triacylglycerol biosynthesis (phosphatidic acid phosphatase, PAP), hydrophobic ... -
Cytokines, alcohol metabolizing enzymes and stress-inducible ER proteins in alcoholic liver disease
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-05-05)The release of inflammatory cytokines by Kupffer cells is believed to be a key step in the pathogenesis of alcoholic liver disease (ALD). Kupffer cells are activated not only by endotoxins [lipopolysaccharides (LPS)] but ... -
Single molecule dynamics
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 2000-03-17)Properties found in an ensemble of molecules may not be understood if it is not possible to study each molecule one by one. This thesis is concerned with the study of the dynamic properties of individual complex biological ... -
Genetic polymorphism of human drug metabolising enzymes : structural and functional studies
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-11-12)There is a pronounced interindividual variability in the levels and activity of many drug metabolising enzymes, which might cause differences in the sensitivity to and the toxicity of many clinically used drugs as well as ... -
Isolation, structure and processing of bioactive peptides
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-11-12)Amino acid sequences deduced from DNA sequences do not prove in which forms proteins, peptides and their processed products are present in the tissues. The products of a single gene may give rise to variants of peptides ... -
Structural and mechanistic aspects of alcohol dehydrogenase function
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-11-05)Vertebrates possess a complex alcohol dehydrogenase (ADH) system composed of multiple molecular forms, which are currently classified into seven classes according to their structural properties. ADHs are dimeric zinc ... -
Leukotriene C4 synthesis and export in human platelets and leukocytes : LTC4 synthase expression in normal and leukemic myeloid cells
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-09-03)Leukotriene (LT)C4 synthase catalyses conjugation of the unstable epoxide LTA4 with reduced glutathione and is the rate-limiting enzyme in the formation of cysteinyl leukotrienes, powerful mediators of asthma and ... -
Genetic polymorphism and regulation of cytochrome P450 2E1
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-08-19)Cytochrome P450 2E1 (CYP2E1) participates in the metabolism of several xenobiotics. A physiological role of CYP2E1 is its ability to convert acetone into gluconeogenetic precursors. Due to a pronounced oxidase activity ... -
The skin barrier : structure and physical function
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-05-04)The objective of the project "The skin barrier - structure and physical function" was to characterise in detail the human skin barrier structurally and functionally. The thesis work includes in-vivo studies, in-vitro studies ... -
Type IV collagen : regulation of COL4A1 and COL4A2 genes and gene therapy of Alport syndrome
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-05-07)Type IV collagen is an important structural protein in basement membranes, which am thin sheet-like structures located between organized tissues and the connective tissue. Type IV collagen is composed of six genetically ... -
Derivatisation of peptides for collision-induced dissociation mass spectrometry
(Institutionen för medicinsk biokemi och biofysik (MBB) / Department of Medical Biochemistry and Biophysics, 1999-03-19)This study is concerned with the mass spectrometric analysis of peptides C-terminally derivatised with aminosulphonic acids. The aim was to increase the sensitivity of detection and to simplify the fragmentation patterns ...