The red blood cell as a mediator of vascular dysfunction in type 2 diabetes and COVID-19
Author: Mahdi, Ali
Date: 2021-11-26
Location: Rolf Luft Auditorium, L1:00, Karolinska Universitetssjukhuset Solna. https://ki-se.zoom.us/j/62990367704?pwd=VWN6ZTFQSS9qZzJWWHBOTjFRUUpQZz09
Time: 09.00
Department: Inst för medicin, Solna / Dept of Medicine, Solna
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Thesis (9.309Mb)
Abstract
Background: The number of patients diagnosed with Type 2 Diabetes (T2D) is steadily increasing and accounts for a large proportion of cardiovascular complications. Endothelial dysfunction represents an early disturbance in the development of atherosclerosis and precedes clinical signs of vascular disease. The mechanisms underlying endothelial dysfunction in T2D are complex but are characterized by decreased bioavailability of the vasodilator nitric oxide (NO) and an imbalance between reactive oxygen species (ROS) and anti-oxidants causing a state of oxidative stress. Arginase has emerged as an important regulator of NO and ROS by consuming the NO substrate L-arginine. Cardiovascular complications arising from coronavirus disease 2019 (COVID-19) have also created a clinical challenge.
Red blood cells (RBCs) have long been considered as innocent nuclei free bystanders, mainly serving the purpose of nutrient transport and gas exchange. Recent evidence has unveiled an important role of these cells in modulating vascular tone through their fascinating ability to release NO bioactivity and their highly developed anti-oxidant defence. Studies have claimed that the structure and function of the RBCs are altered, not only in chronic inflammation as in T2D, but also in acute inflammation in COVID-19. However, the functional implications of these disturbances have not been explored.
Objectives: To explore the importance of dysfunctional RBCs as important mediators of vascular dysfunction in T2D and COVID-19.
Methods and results: In Studies I and II, ex vivo incubations of internal mammary arteries or rat aortic rings with RBCs from patients with T2D resulted in attenuated endothelium-dependent relaxation (EDR) but not endothelium-independent relaxation (EIDR). Mechanistic experiments including expression and enzymatic assays revealed that this impairment was mediated by increased arginase activity and ROS including hydrogen peroxide and peroxynitrite. In Study III, the effect of arginase inhibition in vivo in patients with T2D before and after improvement in glycaemic control was performed using venous occlusion plethysmography. Forearm endothelial dysfunction persisted despite marked improvement in glycaemic control. Two hours of intra-arterial infusion of the arginase inhibitor Nω-hydroxy-nor-L-arginine markedly improved forearm endothelial function both at poor glycaemic control and following four months of improvement in glycaemic control. In Study IV, RBCs were collected from patients with T2D before and after improvement in glycaemic control to assess the impact of dysglycaemia on RBC-mediated endothelial function. The degree of endothelial dysfunction induced by RBCs remained despite clear-cut improvement in glycaemic control. In Study V, in vivo microvascular endothelial function was markedly impaired in patients with COVID-19, both in the acute phase of the infection and at four months follow-up. RBCs from patients with COVID-19 only in the acute phase markedly attenuated both EDR and EIDR through an increase in arginase and ROS and decreased NO bioavailability.
Conclusions: The RBC represents a novel mediator of endothelial dysfunction in patients with T2D (independent of glucose levels) and COVID-19 through alterations in arginase activity and ROS production. Targeting dysfunctional RBCs and the disturbed pathways might represent a previously overlooked therapeutic strategy for improving vascular function and preventing vascular complications in acute and chronic inflammation.
Red blood cells (RBCs) have long been considered as innocent nuclei free bystanders, mainly serving the purpose of nutrient transport and gas exchange. Recent evidence has unveiled an important role of these cells in modulating vascular tone through their fascinating ability to release NO bioactivity and their highly developed anti-oxidant defence. Studies have claimed that the structure and function of the RBCs are altered, not only in chronic inflammation as in T2D, but also in acute inflammation in COVID-19. However, the functional implications of these disturbances have not been explored.
Objectives: To explore the importance of dysfunctional RBCs as important mediators of vascular dysfunction in T2D and COVID-19.
Methods and results: In Studies I and II, ex vivo incubations of internal mammary arteries or rat aortic rings with RBCs from patients with T2D resulted in attenuated endothelium-dependent relaxation (EDR) but not endothelium-independent relaxation (EIDR). Mechanistic experiments including expression and enzymatic assays revealed that this impairment was mediated by increased arginase activity and ROS including hydrogen peroxide and peroxynitrite. In Study III, the effect of arginase inhibition in vivo in patients with T2D before and after improvement in glycaemic control was performed using venous occlusion plethysmography. Forearm endothelial dysfunction persisted despite marked improvement in glycaemic control. Two hours of intra-arterial infusion of the arginase inhibitor Nω-hydroxy-nor-L-arginine markedly improved forearm endothelial function both at poor glycaemic control and following four months of improvement in glycaemic control. In Study IV, RBCs were collected from patients with T2D before and after improvement in glycaemic control to assess the impact of dysglycaemia on RBC-mediated endothelial function. The degree of endothelial dysfunction induced by RBCs remained despite clear-cut improvement in glycaemic control. In Study V, in vivo microvascular endothelial function was markedly impaired in patients with COVID-19, both in the acute phase of the infection and at four months follow-up. RBCs from patients with COVID-19 only in the acute phase markedly attenuated both EDR and EIDR through an increase in arginase and ROS and decreased NO bioavailability.
Conclusions: The RBC represents a novel mediator of endothelial dysfunction in patients with T2D (independent of glucose levels) and COVID-19 through alterations in arginase activity and ROS production. Targeting dysfunctional RBCs and the disturbed pathways might represent a previously overlooked therapeutic strategy for improving vascular function and preventing vascular complications in acute and chronic inflammation.
List of papers:
I. Zhou Z*, Mahdi A*, Tratsiakovich Y, Zahorán S, Kövamees O, Nordin F, Uribe Gonzalez AE, Alvarsson M, Östenson CG, Andersson DC, Hedin U, Hermesz E, Lundberg JO, Yang J, Pernow J. Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I. Journal of the American College of Cardiology. 2018 Aug 14;72(7):769-780. *Equal contribution.
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II. Mahdi A, Tengbom J, Alvarsson M, Wernly B, Zhou Z*, Pernow J*. Red Blood Cell Peroxynitrite Causes Endothelial Dysfunction in Type 2 Diabetes Mellitus via Arginase. Cells. 2020 Jul 16;9(7):1712. *Equal contribution.
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III. Mahdi A*, Kövamees O*, Checa A, Wheelock CE, von Heijne M, Alvarsson M, Pernow J. Arginase inhibition improves endothelial function in patients with type 2 diabetes mellitus despite intensive glucose-lowering therapy. Journal of Internal Medicine. 2018 Oct;284(4):388-398. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Mahdi A, Jiao T, Yang J, Kövamees O, Alvarsson M, von Heijne M, Zhou Z, Pernow J. The Effect of Glycemic Control on Endothelial and Cardiac Dysfunction Induced by Red Blood Cells in Type 2 Diabetes. Frontiers in Pharmacoogy. 2019 Aug 2;10:861.
Fulltext (DOI)
Pubmed
View record in Web of Science®
V. Mahdi A, Collado A*, Tengbom J*, Jiao T, Wodaje T, Johansson N, Farnebo F, Färnert A, Yang J, Lundberg JO, Zhou Z, Pernow J. Erythrocytes induce vascular dysfunction in COVID-19. *Equal contribution. [Submitted]
I. Zhou Z*, Mahdi A*, Tratsiakovich Y, Zahorán S, Kövamees O, Nordin F, Uribe Gonzalez AE, Alvarsson M, Östenson CG, Andersson DC, Hedin U, Hermesz E, Lundberg JO, Yang J, Pernow J. Erythrocytes From Patients With Type 2 Diabetes Induce Endothelial Dysfunction Via Arginase I. Journal of the American College of Cardiology. 2018 Aug 14;72(7):769-780. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
II. Mahdi A, Tengbom J, Alvarsson M, Wernly B, Zhou Z*, Pernow J*. Red Blood Cell Peroxynitrite Causes Endothelial Dysfunction in Type 2 Diabetes Mellitus via Arginase. Cells. 2020 Jul 16;9(7):1712. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
III. Mahdi A*, Kövamees O*, Checa A, Wheelock CE, von Heijne M, Alvarsson M, Pernow J. Arginase inhibition improves endothelial function in patients with type 2 diabetes mellitus despite intensive glucose-lowering therapy. Journal of Internal Medicine. 2018 Oct;284(4):388-398. *Equal contribution.
Fulltext (DOI)
Pubmed
View record in Web of Science®
IV. Mahdi A, Jiao T, Yang J, Kövamees O, Alvarsson M, von Heijne M, Zhou Z, Pernow J. The Effect of Glycemic Control on Endothelial and Cardiac Dysfunction Induced by Red Blood Cells in Type 2 Diabetes. Frontiers in Pharmacoogy. 2019 Aug 2;10:861.
Fulltext (DOI)
Pubmed
View record in Web of Science®
V. Mahdi A, Collado A*, Tengbom J*, Jiao T, Wodaje T, Johansson N, Farnebo F, Färnert A, Yang J, Lundberg JO, Zhou Z, Pernow J. Erythrocytes induce vascular dysfunction in COVID-19. *Equal contribution. [Submitted]
Institution: Karolinska Institutet
Supervisor: Pernow, John
Co-supervisor: Lundberg, Jon; Alvarsson, Michael; Kövamees, Oskar; Yang, Jiangning
Issue date: 2021-11-03
Rights:
Publication year: 2021
ISBN: 978-91-8016-290-6
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