Doktorsavhandlingar / Doctoral Theses - C2 (MBB): Recent submissions

Now showing items 41-60 of 308

    • RNA systems for NMR studies in vitro and in vivo 

      Feyrer, Hannes (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-04-05)
      NMR spectroscopy is an excellent tool to study the structure-function relationship of RNA. Such measurements are usually performed in vitro, which requires large amounts of isotope- labeled sample in high purity and can ...

    • DNA G-quadruplex structures in the mammalian genome : dissecting genome-wide mechanisms of formation and turnover of DNA G-quadruplexes 

      Lyu, Jing (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-03-11)
      Since the beginning of 20th century, when the G-tetrad was first discovered, scientists have been seeking to understand the role of G4 quadruplex structures (G4s) in replication, DNA damage, telomere integrity and the ...

    • Identification of novel modulators of protein synthesis and nucleolar biology using high throughput phenotypic screens 

      Corman, Alba (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-02-23)
      Protein synthesis and ribosome biogenesis are fundamental steps in gene expression and constitute the most energy demanding processes in living cells. Dysregulation of these processes is associated to a variety of human ...

    • Developing and democratizing tools for spatially resolved profiling of genome and transcriptome 

      Gelali, Eleni (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2022-01-21)
      The common thread of the work presented in this thesis is the development and democratization of tools that facilitate the spatial profiling of genome and transcriptome. Paper I describes iFISH, a resource that ...

    • Mitochondrial RNA processing in health and disease 

      Laine, Isabelle (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-12-28)
      Mitochondria are often described as the powerhouses of the cell, providing the main cellular energy source in the form of adenosine triphosphate. Five enzyme complexes, collectively termed the oxidative phosphorylation ...

    • Novel FISH methods to unveil genome architecture 

      Mota, Ana (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-11-22)
      The genome consists of incredibly long DNA strands that encode all the vital information for the cell to function. The DNA inclusion in the very tight nuclear space and, simultaneously, the establishment of a hierarchical ...

    • Developing methods for mapping genetic heterogeneity in tumors : from bulk to single-cell resolution 

      Simonetti, Michele (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-11-15)
      Tumors are complex ecosystems composed of billions of cells that constantly evolve over time. Intra-tumor heterogeneity (ITH) represents the diversity of this complex environment quantifiable both at the genetic and ...

    • Analysis of antibodies to cartilage oligomeric matrix protein in rheumatoid arthritis and in mouse models 

      Cai, Weiwei (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-10-13)
      Rheumatoid arthritis (RA), a common autoimmune disease, affects 0.5-1% of the world’s population. It is not only an important health problem but also a socioeconomic problem considering that it can lead to joint destruction ...

    • Redox regulation of T cells in autoimmunity 

      James, Jaime Rose (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-09-03)
      Autoimmune disorders affect a significant part of the population and therefore present a serious health and economic burden. One of the most common autoimmune diseases is rheumatoid arthritis (RA), affecting 0.5-1% of the ...

    • Positional cloning of polymorphic loci that control autoreactive T cells 

      Aoun, Mike (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-08-13)
      Autoimmune diseases are expensive diseases, both financially and socially, because they are in most cases life long and require a long-lasting treatment. They can also be life threatening, as they are among top 10 causes ...

    • Identifying genetic determinants of T cell-dependent autoimmunity using forward genetics 

      Fernandez Lahore, Gonzalo (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-07-29)
      Rheumatoid arthritis (RA) affects 0.5-1% of the population and is an important health and socioeconomic problem. RA has a high degree of heritability. Thus, extensive efforts have been made to better understand the genetic ...

    • Antibodies as pathogenic factors and biomarkers in rheumatoid arthritis 

      Lönnblom, Erik (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-05-21)
      Ever since the evolution of an adaptive immune system capable of creating immune receptors that may recognize self-antigens, we have been at risk of autoimmunity. There are over 100 different types of autoimmune diseases ...

    • Plurifaceted proteomics in studying cellular dynamics and action mechanisms of anticancer drugs 

      Sabatier, Pierre (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-05-05)
      Mass spectrometry (MS)-based proteomics has developed tremendously in recent years and was the leading technology for many novel methods to study protein chemistry. Contrary to classical approaches based on Western blot, ...

    • Methods development for the investigation of the mammalian genome radial architecture : the quantitative side 

      Girelli, Gabriele (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-04-16)
      The nucleus of mammalian cells cradles the genome, an ensemble of nucleic acid macromolecular polymers that store information in a physical form. For a cell to perform life-sustaining processes, reading and utilizing the ...

    • Spatial regulation of membrane receptor signaling using DNA origami 

      Fang, Trixy (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-04-07)
      Juxtracrine signaling between apposing membrane receptors and ligands is an important class of intercellular communication. Much focus has been directed towards studying the biochemical interactions between receptors and ...

    • Ribosomal RNA dynamics studied by NMR-spectroscopy 

      Karlsson, Hampus (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2021-03-11)
      The ribosome is a large macromolecular machine that consists of both ribosomal proteins and ribosomal RNA (rRNA). It is a complex consisting of two subunits held together by non-covalent interactions, intersubunit bridges ...

    • Cell states along oligodendrocyte development and disease 

      van Bruggen, David (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-12-23)
      The brain, one of the most complex organs in the body, where an immense diversity of cell states emerges from simple structure, where function arises from sets of regulatory principles and pattern persist where individual ...

    • Novel methods for mapping genome organization and genome fragility in the 3D space of the nucleus 

      Kallas, Tomasz (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-11-13)
      For over a century, scientists have been trying to understand how the DNA molecule that is so tightly packed in the micro space of the nucleus sustains critical cellular processes such as transcription, replication or the ...

    • Conformational dynamics in microRNAs : the example of miR-34a targeting Sirt1 mRNA 

      Baronti, Lorenzo (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-06-17)
      In biology, regulatory mechanisms are essential to achieve complex tasks, as virtually every process can be positively or negatively modulated in its outcome, upon different cues. In humans, microRNAs (miRNAs) constitute ...

    • Studies of the thioredoxin system in redox signaling and oxidative stress 

      Espinosa Fernández, Belén (Inst för medicinsk biokemi och biofysik / Dept of Medical Biochemistry and Biophysics, 2020-05-08)
      Reactive oxygen species exert reversible posttranslational modifications in proteins containing redox sensitive thiols, thereby affecting several cellular processes and protein functions. Reductive enzyme systems, such as ...